![]() However, the drawback of sputter coating is it removes the atomic number-contrast and elemental composition analysis. Coating your sample (sputter coating) with an additional thin layer (~10 nm) of a conductive material, such as gold, silver, platinum or chromium, can help remove charging effects and produce better quality images. Consĭue to the nature of the technique and the way it processes samples, a disadvantage of SEM is the fact that it cannot image wet samples as they may be damaged by the vacuum required during operation.Īn SEM is also limited when used to image non-conductive samples and colour images, or when used to take measurements involving height. In short, if the aim of sample imaging is to examine a relatively large area for surface details and composition, SEM is ideal. It’s practical for sample imaging needed for forensic investigations, biological sciences, soil and rock sampling, and medical science. SEM allows for the examination of samples such as metals, alloys and ceramics, as well as polymers and biological materials. Designed for ease of use, the Phenom Pharos is superfast, enabling imaging in less than 30 seconds after sample loading, and it delivers crisp, high resolution imaging (<3nm) with elemental analysis. The Phenom Pharos Desktop Scanning Electron Microscope is an affordable desktop SEM that uses a Field Emission Gun (FEG) source commonly only found in larger, more expensive SEM systems housed in Microscopy centers in major Universities. ![]() As such, it is a key tool for basic research, as well as quality control and failure analysis. The process by which the focused beam of electrons creates a magnified image is so advanced that the magnification is anywhere between 10 and 1,000,000 times. The power of SEM cannot be underestimated. Before deciding on SEM, the sample type and information required should be the top consideration. However, depending on the information required or the type of sample, there are some disadvantages to this method of imaging as well. There are a range of benefits associated with using SEM. What are the Benefits and Disadvantages of SEM As the electrons interact with the sample, they then produce secondary electrons, backscattered electrons and characteristic X-rays. It works to produce images by scanning the sample with a high energy beam of electrons. SEM scans a focused electron beam over a surface to create an image that can be used to obtain information about the surface topography and composition. In this article, we look at SEM imagery and its wide array of uses, as compared to another means of imaging, TEM. On the other hand, TEM uses energetic electrons to provide morphologic, compositional and crystallographic two-dimensional information on samples. Firstly, the detailed, three-dimensional and topographical imaging, and the versatile information this provides, is a big reason why SEM is so effective. While Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) both encompass means of electron imagery, there are some key differences between the two.
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